Evaluation of Multiplex Tandem Real-Time PCR for Detection of Cryptosporidium spp., Dientamoeba fragilis, Entamoeba histolytica, and Giardia intestinalis in Clinical Stool Samples
نویسندگان
چکیده
منابع مشابه
Freezing of stool samples improves real-time PCR detection of Entamoeba dispar and Entamoeba histolytica.
PCR as a tool for intestinal parasite diagnosis is expanding since differentiation between Entamoeba histolytica and Entamoeba dispar cysts is impossible with microscopy. Since pre-analytical factors influence DNA detection, we evaluated with real-time PCR the influence of storage time and temperature. We demonstrated an improved DNA detection in frozen stool samples.
متن کاملPCR detection of Entamoeba histolytica, Entamoeba dispar, and Entamoeba moshkovskii in stool samples from Sydney, Australia.
This study investigated the presence of Entamoeba histolytica, Entamoeba dispar, and Entamoeba moshkovskii in stool samples from a patient population in Sydney, Australia. Stool samples were tested by microscopy and PCR. Five patients were found with E. histolytica infections, while E. dispar and E. moshkovskii were observed in 63 (70.8%) and 55 (61.8%) patients, respectively, by PCR. This is t...
متن کاملReal-time PCR for detection and differentiation of Entamoeba histolytica and Entamoeba dispar in fecal samples.
A closed-tube, real-time PCR assay was developed for sensitive and specific detection and differentiation of the two closely related intestinal protozoan parasites Entamoeba histolytica and Entamoeba dispar directly from human feces. The assay is performed with the LightCycler system using fluorescence-labeled detection probes and primers amplifying a 310-bp fragment from the high-copy-number, ...
متن کاملapplication of multiplex pcr for detection and differentiation of entamoeba histolytica, entamoeba dispar and entamoeba moshkovskii.
background: entamoeba moshkovskii and e. dispar are impossible to differentiate microscopically from the pathogenic species e. histolytica . multiplex polymerase chain reaction (multiplex pcr) is a widespread molecular biology technique for amplification of multiple targets in a single pcr experiment. methods: for detection and differentiation of the three-microscopy indistinguishable entamoeba...
متن کاملEvaluation of three diagnostic methods, including real-time PCR, for detection of Dientamoeba fragilis in stool specimens.
Dientamoeba fragilis is a protozoan parasite of humans that infects the mucosa of the large intestine and is associated with gastrointestinal disease. We developed a 5' nuclease (TaqMan)-based real-time PCR assay, targeting the small subunit rRNA gene, for the detection of D. fragilis in human stool specimens and compared its sensitivity and specificity to conventional PCR and microscopic exami...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 2010
ISSN: 0095-1137
DOI: 10.1128/jcm.01796-10